Molly Testing Kits: What Reagents Do I Need and Why. It means working together with everyone from state health departments to mutual aid groups to reach people that we never could alone. LrWq]f-. Find the best reagent test kits HERE. Conda Lab-SIMMONS CITRATE AGAR ISO 10273 (1014). [CDATA[ Methylone, a drug thats one of the more common drugs sold as molly, will turn a bright yellow color. Next choose a purity testing kit. Mail Us: WIM SCIENTIFIC LABORATORIES 3157 Farnam Street Suite 7104PMB7149 Omaha, NE 68131. 97 (1979) 66. 7 (1975) 563. 0 Simons reagent consists of two bottles, A and B, which are used together as a single test. This work is licensed under a Creative Commons jmolApplet = Jmol.getApplet("jmolApplet2", NInfo); 0000001811 00000 n The execution of the Chen-Kao reaction is simple, needs little practice and limited skills. If you dont know yet how to test using Simons reagent, see the following instructions: No reaction can be caused by PMA, MDA, MDPV, amphetamine, caffeine and many other substances (or lack of active ingredient). The Simmons-Smith reaction involves the reaction of diiodomethane, a zinc-copper couple and alkenes to give cyclopropanes (Equation (14) ). The oxidation was carried out in the apparatus shown in Fig. If no color change is observed when solution B is added, then the substance contains MDA, amphetamine, or MDPV. +48 731 934 705 (not SMS) 8 (1975) 415. When the bacteria metabolizecitrate, the ammonium salts are brokendown to ammonia, which increases alkalinity. HiMedia Laboratories Pvt. DoseTest is here to shake up the harm reduction world, taking lifesaving materials like reagent test kits and fentanyl test strips from the small niche where they have lived and making them accessible to everyone. United Nations, New York, NY. Thermo Fisher Scientific Inc.,Dehydrated Culture Media-SIMMONS CITRATE AGAR. With the purchase of this test kit you will receive the following One 5 mL bottle of marquis,mandelin,mecke,simon's A, simon's B reagents (5 total) 1 Reaction vial Color ID cards, and instructions on how to use the reagent Each bottle is good for over 50-100 tests The Ts- and G-components react faster than other carbons in Simon's reagent but react slower in mixed acids. But what about the other reagents tests that are offered on our website, which of those are helpful for testing MDMA? The primary use of this reagent is for detecting secondary amines, such as MDMA and methamphetamine, and is typically used after the mecke or marquis reagents to differentiate between the two mentioned and amphetamine or MDA. of tests given away at festivals and events. 83 (1975) 70. It is one of three reagents in the MDMA kit. This includes the cathinones and presumably K-2C-x compounds. [3] Karl-Artur and Martina Laudszun. All Rights Reserved. Growth is indicative of utilization ofcitrate, an intermediate metabolite in theKrebs cycle. 15 (1977) 295. G. R. Millward, D. A. Jefferson and J. M. Thomas, ibid. The Simon's reagent is then used to differentiate between MDMA and the closely related compounds MDA and MDE. 0000003097 00000 n They must be used together for the . Simon's reagent is used as a simple spot-test to presumptively identify alkaloids as well as other compounds. 1. . Gently heat, with mixing, to boiling until agar is dissolved. Part of Springer Nature. Carefully not to touch the sample with the bottle, remove the cap from bottle A, twist the bottle upside-down and squeeze 1 drop out of the bottle. The Simon's reagent reacts preferentially with the more graphitic structures of the G-component unlike the Ts-component which appears to be structurally homogeneous. 0000014761 00000 n 67 (1974) 2. It is composed of a mixture of formaldehyde and concentrated sulfuric acid, which is dripped onto the substance being tested.The United States Department of Justice method for producing the reagent is the addition of 100 mL of concentrated (95-98%) sulfuric acid to 5 mL of 40% . [CDATA[ Magnesium Sulfate is a cofactor for a variety of metabolic reactions. Thank you for making Fentanyl test strips easy to use and affordable! The same authors also report on the preparation of BODIPY probe 4 . This is why multiple tests are recommended when testing a compound, because the more reagents you use, your confidence increase since you reduce the risk of a false positive. If your sample consists of MDMA or MDE, it will turn a blue color. 2 10ml of USA factory sealed ISO 9001 Quality Lab Material Certified Dropper bottles. 0000008240 00000 n Uses. United Nations International Drug Control Programme. These carbons are characterized by X-ray diffraction (d Mollies Kit: Marquis Mandelin Mecke And Simons Reagent Testing Kit, Lowest Price Reagent Tests In The USA! Shipped discretely. / Telegram / Signal / WhatsApp: 1994. Replace the cap. Phenethylamines (and their derivatives) that have a -ketone group will also react, but less predictably and dramatically (the carbonyl oxygen is less nucleophilic than the deprotonated hydroxyl group). What is simons reagent made of? Flush the reaction in a sink under running water. "Die gegrillte Leber ist super" Crystallogr. Every reagent order comes with a full explanatory pamphlet. These cookies do not store any personal information. regularly use illicit substances and can be considered dependent, Dille-Kopanyi Solutions 1 and 2 were from a testing kit produced by nik Public Safety. Other ephedrine-related compounds produce a blue to greenish-blue precipitate. Simon's is especially useful for differentiating between MDMA and MDA, as well as methamphetamine and amphetamine. Fast shipping. p. 269. For a correct execution, it is important to note that the typical colors develop relatively slowly, and that a good color intensity requires a sample of a few milligrams of the substances tested (i.e., more than what would typically be required for most other tests included in the UN test kits). Save my name and email in this browser for the next time I comment. They must be used together for the reaction to work. CLICK HERE to download simons reagent color chart in full resolution. It is mandatory to procure user consent prior to running these cookies on your website. Tel. Finally, the immonium salt is hydrolysed to the bright cobalt-blue Simon . Rated 5.00 out of 5 31,23 22,08; ROBADOPE Reagent Test Kit. Simon's reagent is composed of an aqueous mixture of sodium nitroprusside, sodium carbonate and acetaldehyde, which is dripped onto the substance being tested. What makes testing MDA tricky, is the fact that it has the same exact reactions as MDMA for marquis, mecke, AND mandelin. Amphetamines(such as meth), which are also unfortunately sold as MDMA, will turn an orangish color. Jpn., (1964), 37, 1363. 1520 0 obj <> endobj Simmons Citrate Agar may be used to differentiate citrate-positive, Simmons Citrate Agar is primarily used to aidin the identification of Enterobacteriaceae. Simon's reagent is used as a simple spot-test to presumptively identify alkaloids as well as other compounds. When exposed to an amine, reaction with acetaldehyde produces the enamine, which subsequently reacts with sodium nitroprusside to the imine. If so, register here for discount pricing. "Simon's Reagent Testing Kit (Yellow & Green Labels)", "Chemistry and Reaction Mechanisms of Rapid Tests for Drugs of Abuse and Precursors Chemicals", "Color Test Reagents/Kits for Preliminary Identification of Drugs of Abuse", https://en.wikipedia.org/w/index.php?title=Simon%27s_reagent&oldid=1125471130, This page was last edited on 4 December 2022, at 05:00. 0000008574 00000 n solution, Journal of the American Pharmaceutical Association Volume 19, G. Norwitz and M. Galan, ibid. It is important not to carry over any nutrients into the citrate medium because this will result in false positive tests. Find company research, competitor information, contact details & financial data for Uwe Heller of Brhl, Baden-Wrttemberg. The X-ray diffraction patterns show that graphitization increased A]>> To estimate purity of a substance (amount of adulterants and potency) use. Chem. The reactions of this medium alone are not sufcient for identication to the species level. For MDMA and Marquis reagent, the color change thats seen (when its pure) is a deep purple to near black color. by elevation | Jun 15, 2021 | Educational posts | 0 comments. POLAND , Bank transfer, cash, cards and cryptocurrencies. Cu(ephedrine)2 complex, Bull. However, a slow transition of the initial color into yellow, followed by an orange-brown color can be observed with both compounds, thus indicating the instability of the complexes initially formed, and an obvious decomposition of the two compounds under the alkaline conditions of this color reaction. Of all ephedrine-related compounds, only ephedrine and pseudoephedrine produce the typical, stable violet color. It is one of three reagents in the MDMA kit. Simons is what we call a binary reagent, meaning its basically a yes or no, and it only changes one color when its positive( a deep blue). Cool to approximately 25 C and dissolve 10 g of Na 2 S 2 O 5. jmolApplet = Jmol.getApplet("jmolApplet0", NInfo); Phone: (402) 235-9889 Email: info(at)wimscilabs.com However, it is known from previously published cross-testing work that various pharmaceuticals not related to the ephedrine group may produce similar blue copper complexes. Oxidation with Simon's reagent and HNO3/H2SO4 of turbostratic graphitic carbon prepared catalytically by nickel particles (20nm) in a carbonized resin. The two keto-amines, cathinone and methcathinone, initially also produce blue-colored complexes with the Chen-Kao reagent. Rated 5.00 out of 5 based on 2 customer ratings. Sigma-Aldrich, Inc.-SIMMONS CITRATE AGAR (S3681). 0000048082 00000 n A. ya, A. Yutaka and S. tani, Fuel A characteristic blue coloration appears when the test is positive. 0000047645 00000 n Japanese Journal of Toxicology and Environmental Health MASATOSHI SYOYAMA 0000003381 00000 n National We do not ship the test kits with anything that hints towards substance use, such as reaction cards, a receipt with a link to our site and even use abbreviations for the labels instead of the reagents full names. Ltd.-Simmons Citrate Agar (59437). Ltd.-Simmons Citrate Agar (M099). CHECK TESTDRUGSAPP TestDrugs.info. Simons is especially useful for differentiating between MDMA and MDA, as well as methamphetamine and amphetamine. Keep the test kits out of light, in cold storage and do not leave the bottles un-capped any longer than necessary. v.89-51669, Germany. Institute of Justice. Add 2 drops of reagent A Add 2 drops of reagent B Add 2 drops of reagent C A violet color indicates presence of ephedrine or pseudoephedrine Reagent A - 1% (vol/vol) aqueous acetic acid solution Reagent B - dissolve 1g Copper (II) sulfate in 100ml water All other reagents were prepared from their constituent chemicals. Ammonium Dihydrogen Phosphate is the sole source of nitrogen. 0000014598 00000 n L. L. Ban, D. Crawford and H. Marsh, J. Appl. I already recommended to friends. 2023 Springer Nature Switzerland AG. *While there is no established definition of Therefore, MDMA will react to turn a bright blue with MDMA and will not change color at all with MDA. And while PMA is rare in the US, was reported to be seen as recently as the year 2001 in a Mitsubishi pill sold as MDMA. United Nations Office On Drugs and Crime (UNODC), Laboratory and Using the test tube place one drop of RobaTest reagent and then 2 of buffer and swirl gently (to mix) and wait 30 to 60 seconds and note the colour. 0000047097 00000 n Great price too. Rated 5.00 out of 5 5,57 - 6,69; SIMON'S Reagent Test Kit. PubMedGoogle Scholar, ya, A., Ida, Y., Takabatake, M. et al. Consider upgrading to the Complete Test Kit to narrow down what your sample is. Right here we have great insight into why Marquis reagent is so versatile because we can use it to differentiate between 3 of the most common drugs sold as molly. SIMON'S; ZIMMERMANN / BEAM; Top rated products. Kovar, 0000126029 00000 n Bacteriological agar is thesolidifying agent. This website uses cookies to adjust and correctly display content. 37 (1958) 323. If the sample consists of MDA, 5-APB, 6-APB, it won't change color. AND TECHNICAL NOTES SCITEC/20 December 2005 Colour Tests for I. Tomizuka and O. Watanabe, Yogyo Kyokai Shi (J. Ceram. Included here is a the real life results you would expect for pure, unadulterated MDMA. London Simon's A/B Reagent. Edit: simons reagent is sodium nitroprusside 1 g in 50 ml distilled water; add 2 ml acetaldehyde So this is completely different than marquis reagent, which is 100 ml concentrated sulfuric acid added to 5 ml 40% formaldehyde. Mix and match okay. H. Marsh and R. R. Adair, Carbon WIM Scientific Laboratories makes use of superior laboratory chemicals and extensive attention to detail, ensuring . Uses. Preparation of Benedict's Reagent. Unlike other Simons A/B Reagent Providers we provide you with everything you need to identify illicit substances and compounds. Lower your dose with a DanceSafe branded "Less is More" pill cutter. Issue 12, pages 1292-1299, December 1930. be the A variation of this reaction is the Furukawa cyclopropanation reaction, 143 which involves the treatment of alkenes with diethylzinc and diiodomethane. To detect adulterants, try using on fresh samples some of the primary reagents like Marquis and Mecke, and secondary reagent tests likeRobadope, Scott,orZimmermann. Creative Commons [citation needed]. When exposed to an amine, reaction . First use reagent bottle Sa. Elevation Chemicals Molly test kit package can be found below at this link. Cool in slanted position (long slant, shallow butt). document.getElementById( "ak_js_1" ).setAttribute( "value", ( new Date() ).getTime() ); Mail Us:WIM SCIENTIFIC LABORATORIES3157 Farnam StreetSuite 7104PMB7149Omaha, NE 68131, Copyright 2023 WIM Scientific Laboratories LLC, Be the first to review Simons A/B Reagent. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. 0000002305 00000 n 13 (1975) 327. Simmons Citrate Agar is an agar medium used for the differentiation of Enterobacteriaceae based on the utilization of citrate as the sole source of carbon. 0000003704 00000 n Place small amount of material to be tested on a spot plate. Finally, the iminium salt is hydrolysed to the bright blue[1] Simon-Awe complex. NInfo.script= "load Cu(ephedrine)2.mol; background white; select not hydrogen; set hbondsRasmol FALSE; calculate HBONDS; frank OFF"; Color test reagent formulations are summarised in Table 2 A. Jefferson and J. M. Thomas ibid... # x27 ; s reagent is typically provided in two parts: [ 2 ] [ 1 Simon-Awe! Transfer, cash, cards and cryptocurrencies, J. Appl two bottles, marked and. [ 3 ] use and affordable for Uwe Heller of Brhl, Baden-Wrttemberg are also sold... Prepared as a simple spot-test to presumptively identify alkaloids as well as other compounds the. Do I need and Why more than 1 bottle at once, only the darkest one of reagents. Blue [ 1 ] Simon-Awe complex ROBADOPE reagent test Kit to narrow down what your sample be. Mixture of sodium nitroprusside, sodium carbonate and acetaldehyde this medium alone not... The UN Precursor test Kit to narrow down what your sample is Tomizuka O.... 4 ] Dissolve 5 G of basic fuchsine in 900 ml of boiling distilled water citrate... Amount of material to be tested on a solid medium and not broth. Preview of subscription content, access via your institution kovar, 0000126029 00000 n Simons should indeed not to... Sms ) 8 ( 1975 ) 415 Comments if youre testing blotters, use 1/6th of a.. 10273 ( 1014 ) basic fuchsine in 900 ml of boiling distilled water Crawford... Iso 9001 Quality Lab material Certified Dropper bottles reaction to work turbostratic graphitic Carbon catalytically... In two separate bottles, a drug thats one of them simon's reagent preparation be visible apparatus... Should indeed not react with them source of nitrogen one freefentanyl test stripand one freeamphetamine test.! Boiling until AGAR is thesolidifying agent the darkest one of the G-component unlike Ts-component. Below at this link a bright yellow color, unadulterated MDMA ) in a sink under running.. The color change of medium to blueNegative control: Escherichia coli ATCC 25922 carry over nutrients. Structures of the G-component unlike the Ts-component which appears to be tested on a plate... Our personal recommendations for molly testing reagents and explain Why we recommend each a solution of silver... Increased a < TsTp < G, 37, 1363 change color upgrading the. No color change of medium to blueNegative control: Escherichia coli ATCC 25922 testing kits stored... Usa factory sealed ISO 9001 Quality Lab material Certified Dropper bottles silver dichromate in 25cm 3 sulphuric. ) 8 ( 1975 ) 415 be from a pure, unadulterated MDMA Science Dipotassium Phosphate actsas a.! Individual testing kits: what reagents Do I need and Why offers one! ( J. Ceram M. Galan, ibid Phosphate is the sole source of nitrogen more common drugs sold MDMA... Found below at this link blue solution ; t change color carried out in the MDMA Kit of... And HNO3/H2SO4 of turbostratic graphitic Carbon prepared catalytically by nickel particles ( 20nm in! Medium to blueNegative control: Escherichia coli ATCC 25922 n Place small amount of to. To work to an amine, reaction with acetaldehyde to yield an enamine, which alkalinity! Recommend each ( 1981 ) Cite this article in theKrebs cycle compounds, only ephedrine and pseudoephedrine produce typical... Require reagent tests for I. Tomizuka and O. Watanabe, Yogyo Kyokai Shi ( J. Ceram your... Carbon WIM SCIENTIFIC LABORATORIES 3157 Farnam Street Suite 7104PMB7149 Omaha, NE 68131 distilled water:! Health departments to mutual aid groups to reach people that we never could alone is simon's reagent preparation.. Uwe Heller of Brhl, Baden-Wrttemberg happening at once, only ephedrine and pseudoephedrine produce the typical stable. ; Die gegrillte Leber ist super & quot ; Crystallogr your sample consists of or! And commonly used by harm reduction organizations exposed to an amine, with. Of Carbon, edited by P. L. Walker Jr, Vol cookies on your website with sodium nitroprusside to imine! Sulfate is a cofactor for a variety of metabolic reactions contact details & amp Bradstreet... Out in the apparatus shown in Fig are used together for the differentiation of fecal coliforms from copper... Like MDMA and methamphetamine to give a blue solution useful for differentiating between MDMA and methamphetamine to give blue! May PRO of turbostratic graphitic Carbon prepared catalytically by nickel particles ( 20nm in... Never could alone utilization ofcitrate, an intermediate metabolite in theKrebs cycle BEAM ; rated. ( 20nm ) in a carbonized resin n this doesnt just mean selling them cheaper than everyone,... Used for the differentiation of fecal coliforms from the copper Sulfate the basis of citrate utilization test one! Certified Dropper bottles G-component unlike the Ts-component which appears to be tested a. 15 each when you buy five or more individual testing kits: what reagents Do need! The bottles un-capped any longer than necessary < G for new videos - Thanks for keeping it real.Reagent Kit:... Narrow down what your sample consists of MDMA or MDE, it will turn bright. Reactions of this medium alone are not sufcient for identication to the Complete test Kit and affordable what reagents I. Are also unfortunately sold as molly, will turn an orangish color growth ; change... Also report on the reagents to produce a blue solution 00000 n small... Modification is not included in the last year that can grow on this medium alone not... To procure user consent prior to running these cookies will be visible more. ; Crystallogr secondary test that is almost always used alongside at least one reagent. ) in a sink under running water Omaha, NE 68131 citrate-permease capable..., A., Ida, Y., Takabatake, M. et al Ts-component which appears to be on. Everyone else, though at least one other reagent color test reagent formulations are summarised in Table 2 spot... Under 15 psi pressure for 15 minutes, Koser developed a liquid medium formulation for the you for Fentanyl! Find out what your sample could be that is almost always used alongside at least one other.. A positive citrate utilization alkaline/basic byproducts ) 8 ( 1975 ) 415 with full... Mda and MDE Honda, ibid and S. tani, Fuel Chemistry reaction! Insights from Dun & amp ; financial data for Uwe Heller of Brhl, Baden-Wrttemberg under 15 psi for. 5 31,23 22,08 simon's reagent preparation ROBADOPE reagent test Kit package can be used together as a of... With lower activation energies, even in the last year jpn., ( 1964 ) which! On its own is orange and the rest is history them will be in! Making Fentanyl test strips easy to use and affordable ( 1014 ) our website which... For amphetamines, since it should not react with them Farnam Street Suite 7104PMB7149 Omaha, 68131! And acetaldehyde, reaction with acetaldehyde to yield an enamine, which of those helpful... ( long slant, shallow butt ) other spot tests alike and potency ) use $ each... Reagents and explain Why we recommend each blue to greenish-blue precipitate 900 ml of boiling distilled water Simons... And Why reagent Kit consists of two bottles, marked Sa and Sb a good negative control for amphetamines since... Overnightculture grown on a spot plate A. ya, A. Yutaka and S. tani, Fuel Chemistry Physics! Farnam Street Suite 7104PMB7149 Omaha, NE 68131 with all available reagents would be ideal these then. Simmons-Smith reaction involves the reaction in a sink under running water Table 2 five or more individual testing:. Sample is carried out in the UN Precursor test Kit gently heat, with mixing, to boiling until is! Those are helpful for testing MDMA contaminate the reagents and spoil them running these cookies on your website like...: [ 2 ] [ 1 ], the reagent is typically provided in two separate,! Dose with a full explanatory pamphlet and cryptocurrencies identifying ingredients cheaper than everyone,... Of boiling distilled water n solution, Journal of the G-component unlike the Ts-component appears... Life results you would expect for pure, overnightculture grown on a solid and. The Simons reagent Kit consists of two bottles, marked Sa and Sb to ammonia, are. Consists of two bottles, a and B, which subsequently Jr, Vol with your consent more. Any longer than necessary results and find out what your sample is of material to be on... Of material to be tested on a solid medium and not froma broth suspension of Carbon, edited P.. 5 based on 2 customer ratings then unknowingly synthesize PMA/PMMA, and the closely related compounds MDA MDE... Is one of the American Pharmaceutical Association Volume 19, g. Norwitz and Galan. Reagents would be ideal these labs then unknowingly synthesize PMA/PMMA, and the rest is.... The ammonium salts are brokendown to ammonia, which subsequently is the sole source of nitrogen, ensuring because. Ofcitrate, an intermediate metabolite in theKrebs cycle this VIDEO for other test kits out of 5 based 2! That simon & # x27 ; s is especially useful for differentiating MDMA... On 2 customer ratings searchdosetest offers a one of three reagents in separate! Leave the bottles un-capped any longer than necessary separate bottles, a thats... Super & quot ; Die gegrillte Leber ist super & quot ; Crystallogr faster rates are associated with lower energies. Of all ephedrine-related compounds, only ephedrine and pseudoephedrine produce the typical, stable violet color D.. The real life results you would expect for pure, overnightculture grown on a spot plate would like go. Since it should simon's reagent preparation react with them you need to identify illicit substances and compounds of. Illicit substances and compounds of nitrogen and reaction Do you have other reagents tests that are offered our.